Toluidine blue staining of murine mast cells and quantitation by a novel, automated image analysis method using whole slide skin images

Source : https://doi.org/10.1080/01478885.2021.1915934

ABSTRACT Mast cells are immune cells of myeloid lineage, characterized by the presence of cytoplasmic granules. These cells play a significant role in multiple pathologies, such as urticaria and type 1 hypersensitivity reactions. Mast cells in tissue sections can be demonstrated with metachromatic stains, such as toluidine blue.